产品货号：
GS2176
中文名称：
BstBAI内切酶
英文名称：
BstBAI
产品规格：
250U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
相关搜索：BstBAI内切酶，BstBAI限制性内切酶
使用说明
To obtain 100%activity,BSA should be added to the 1x reaction mix to a final concentration of 100μg/ml.Do not use BSA for long incubation.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | YAC↑GTR |
| 来源 | Bacillus stearothermophilus BA |
| Assayed on | Lambda DNA |
| Optimal buffer | W(10mMTris-HCl(pH 8.5 at 25℃);10mMMgCl2;100 mM NaCl;1 mM DTT.)+ BSA |
| Optimal temperature | 65℃ |
| Storage conditions | 10mMTris-HCl(pH 7.5);50 mM KCl;0.1 mM EDTA;10mM2-mercaptoethanol;200 μg/ml BSA;50%glycerol.Store at -20℃. |
| Ligations | After 10-fold overdigestion with enzyme more than 90%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a.of enzyme for 16hoursat 65℃. |
| Methlation effect | Blocked by CG methylation. |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | BsaAI,Ppu21I |
相关搜索：BstBAI内切酶，BstBAI限制性内切酶